Open AccessAnalysis of the genome of the five Bifidobacterium breve strains: plasmid content, pulsed‐field gel electrophoresis genome size estimation and rrn loci number
Author(s) -
Bourget Nathalie,
Simonet JeanMarc,
Decaris Bernard
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06288.x
Subject(s) - restriction enzyme , bifidobacterium breve , plasmid , genome , biology , strain (injury) , gel electrophoresis , microbiology and biotechnology , genome size , restriction map , endonuclease , pulsed field gel electrophoresis , genetics , dna , gene , bifidobacterium , bacteria , genotype , anatomy , lactobacillus
The genomes of the five Bifidobacterium breve strains available from culture collections were compared by restriction endonuclease analysis. Electrophoretic migration of undigested DNA allowed us to detect a 5.6‐kb circular plasmid in two of these strains. A restriction map of this plasmid was constructed using 10 enzymes. With Dra I endonuclease, pulsed‐field gel electrophoresis has allowed the determination of the five B. breve genome sizes to 2.1 Mb. This estimation was further confirmed for CIP 6469 (type strain) and ATCC 15698 using Xba I and Spe I enzymes. In addition, rRNA gene regions were used as probes for strain characterization and suggest that there are at least three rrn loci in B. breve .