Open AccessProduction of heat‐stable enterotoxin II by chicken clinical isolates of Escherichia coli
Author(s) -
Akashi Noriko,
Hitotsubashi Shunji,
Yamanaka Hiroyasu,
Fujii Yoshio,
Tsuji Takao,
Miyama Akio,
Joya Josephine Encarnacion,
Okamoto Keinosuke
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06186.x
Subject(s) - enterotoxin , escherichia coli , heat stable enterotoxin , enterotoxigenic escherichia coli , diarrhea , microbiology and biotechnology , antiserum , biology , polymerase chain reaction , enterobacteriaceae , chemistry , antigen , gene , biochemistry , medicine , immunology
Enterotoxigenic Escherichia coli isolated from diarrhea stools of chickens were examined for production of heat‐stable enterotoxin II which is considered to be implicated only in diarrhea of pigs. Seven out of 38 strains examined were found to contain heat‐stable enterotoxin II gene, determined by colony hybridization and the polymerase chain reaction. The culture supernatants of these strains caused fluid accumulation in the mouse intestinal loop test. This fluid accumulation activity was not lost by heating at 100°C and was neutralized by anti‐heat‐stable enterotoxin II antiserum. Purified heat‐stable enterotoxin II caused fluid accumulation in the chicken intestinal loop assay. These results indicate that STII‐producing E. coli is implicated in chicken diarrhea.