Open AccessLarge scale production and purification of human retrovirus‐like particles related to the mouse mammary tumor virus
Author(s) -
Faff Ortwin,
Murray Beatrice A.,
Erfle Volker,
Hehlmann Rüdiger
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06183.x
Subject(s) - retrovirus , mouse mammary tumor virus , centrifugation , differential centrifugation , ultracentrifuge , cell culture , virus , virus like particle , biology , human breast , microbiology and biotechnology , chemistry , virology , cancer , cancer cell , biochemistry , gene , recombinant dna , genetics
Human retrovirus‐like particles related to mouse mammary tumor virus (MMTV) are secreted in a steroid‐dependent manner by the breast cancer cell line T47D. We report the successful large scale production and purification of these particles from culture supernatants of T47D cells and describe the experimental conditions established for this purpose. Thus, mg amounts of particles were produced by large scale culturing of T47D cells in an autoharvesting roller bottle system and purified by differential centrifugation and continuous flow ultracentrifugation on density gradients with a 50% recovery and a 350‐fold enrichment.