Open AccessUse of Tn 4560 to generate nikkomycin non‐producing mutants of Streptomyces tendae
Author(s) -
Engel Paul,
Wright Maureen S.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06177.x
Subject(s) - mutant , transposable element , streptomyces , biology , bioassay , streptomycetaceae , actinomycetales , gene , high performance liquid chromatography , strain (injury) , microbiology and biotechnology , chemistry , genetics , bacteria , chromatography , anatomy
Transposon Tn 4560 was used to generate three nikkomycin non‐producing mutants in Streptomyces tendae ATCC 31160 Southern hybridization confirmed that Tn 4560 was present in 10–12‐kb Bam HI fragments of the chromosomes of the mutants. Biologically active nikkomycins were not detected in culture broths of the mutants as determined by bioassays and HPLC. Differences in the HPLC profiles of culture broths suggest that Tn 4560 inserted into different genes in the mutants.