Open AccessCharacterization of a small cryptic plasmid from Salmonella enteritidis that affects the growth of Escherichia coli
Author(s) -
Ibáñez Magdalena,
Rotger Rafael
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06172.x
Subject(s) - plasmid , salmonella enteritidis , replicon , extrachromosomal dna , biology , escherichia coli , microbiology and biotechnology , enterobacteriaceae , restriction enzyme , pbr322 , plasmid preparation , salmonella , genetics , dna , bacteria , gene
We examined the plasmid content of 25 clinical isolates of Salmonella enteritidis , and detected the presence of small plasmids (3–5.3 kb) in 9 of them, alone, or in addition to the large, so‐called virulence plasmid. A 5.3‐kb plasmid isolated as unique extrachromosomal DNA from a strain responsible for a high‐mortality outbreak was characterized by restriction mapping and cloning. The plasmid replicon was localized in a 1.7‐kb fragment, that hybridized with three of the small plasmids detected in S. enteritidis , and with another small plasmid from Salmonella typhimurium . A strain of Escherichia coli carrying this plasmid, or a cloned 3.7‐kb Pvu II restriction fragment, showed a slower growth rate, especially in minimal medium, as well as a noticeable increase in DNA methyltransferase activity.