Open AccessTwo Streptomyces lividans 66 transfer RNA genes with anticodons corresponding to serine (AGC) and arginine (CGU) codons
Author(s) -
Ulrix W.,
Steen G.,
Aert R.,
Robben J.,
Volckaert G.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06170.x
Subject(s) - transfer rna , genetics , wobble base pair , terminator (solar) , biology , gene , open reading frame , aminoacylation , rna , peptide sequence , ionosphere , physics , astronomy
From the nucleotide sequence of a 2110‐bp Streptomyces lividans 66 DNA fragment two transfer RNA genes were identified: tRNA Ser (AGC) and tRNA Arg (CGU). These tRNA genes are transcribed from the same DNA strand and both are preceded by a putatife promoter structure. They are separated by an intergenic region of 191 bp. Like most Streptomyces tRNA genes described so far, they do not encode the 3′ terminal CCA of mature tRNAs. Both genes are followed by extensive inverted repeats, which could serve as transcriptional terminator signals. Remarkably, these hairpin structures share an identical 9 base pair stretch (5′‐GAAGCCCCG‐3′). Furthermore, the tRNA Arg region is followed by two potential open reading frames, which are encoded on complementary strands and have 3′ overlapping ends. The gene for tRNA Arg (CGU) is the first Streptomyces tRNA gene described so far which encodes the translation of a codon with uridine at the third (wobble) position.