Open AccessSynchronous germination of Streptomyces antibioticus spores: Tool for the analysis of hyphal growth in liquid cultures
Author(s) -
Miguélez Elisa M.,
Martín Cruz,
Hardisson Carlos,
Manzanal Manuel B.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06156.x
Subject(s) - hypha , spore , germination , liquid culture , elongation , spore germination , liquid medium , streptomyces , microbiology and biotechnology , botany , biology , chemistry , chromatography , materials science , bacteria , composite material , genetics , ultimate tensile strength
We have devised a method for obtaining synchronous and dispersed growth of Streptomyces antibioticus in liquid cultures. After ultrasonic treatment, most of the spores germinated at the same time, yielding hyphae very similar in length. Dispersed growth was achieved in media without Ca 2+ and in which the levels of Fe 2+ and Mg 2+ were carefully controlled. Studies on the kinetics of growth carried out with synchronous cultures of young hyphae revealed a multiphasic pattern of hyphal elongation, with successive periods of linear growth and changes in growth rate at defined intervals.