Open AccessCompatible solutes in representatives of the genera Brevibacterium and Corynebacterium : Occurrence of tetrahydropyrimidines and glutamine
Author(s) -
Frings Eric,
Kunte H.Jörg,
Galinski Erwin A.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06138.x
Subject(s) - brevibacterium , osmolyte , betaine , corynebacterium , glutamine , biology , biochemistry , bacteria , corynebacterium glutamicum , ectoine , microbiology and biotechnology , osmoprotectant , proline , amino acid , microorganism , genetics , gene
The aim of our investigation was to study the haloadaptation of a number of species of Gram‐positive bacteria belonging to the genera Brevibacterium and Corynebacterium . We used two different HPLC‐techniques and 13 C‐NMR spectroscopy for the identification of osmolytes (compatible solutes). The tetrahydropyrimidines (‘ectoines’) are the main compatible solutes in the genus Brevibacterium , whereas accumulation of glycine‐betaine and accumulation of synthesis of glutamine is mainly responsible for osmoadaptation in the genus Corynebacterium . Pipecolic acid, formerly described as a potential osmolyte synthesized de novo in C. ammoniagenes , does not contribute markedly to the solute pool, unless supplemented to the medium.