Open AccessDiversity in nucleotide acquisition by antigenically similar Chlamydia psittaci of avian origin
Author(s) -
McClarty Grant,
Fan Huizhou,
Andersen Arthur A.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06123.x
Subject(s) - chlamydia psittaci , biology , restriction enzyme , thymidine , genotyping , virology , serotype , polymerase chain reaction , microbiology and biotechnology , nucleotide , chlamydia , dna , genotype , gene , genetics
Two different nucleic acid precursor utilization patterns were obtained for five avian isolates of Chlamydia psittaci . Three of the isolates bahaved in a manner similar to that previously described, showing total dependency on the host cell for ribonucleoside triphosphates and being unable to utilize medium‐supplied thymidine. In contrast, the other two isolates were incapable of taking pyrimidine ribonucleotides from the host cell and they could efficiently utilize medium‐supplied thymidine. These unusual isolates were resistant to 5‐fluorouridine while the other three isolates were sensitive. Of the five isolates only 6BC was sensitive to sulfonamides. The five isolates were divided into two groups by comparing the Alu I restriction endonuclease patterns obtained following digestion of the major outer membrane protein (OMP1) gene, amplified by the polymerase chain reaction. The OMP1 genotyping results were confirmed by serotyping.