Open AccessLysogenic phage in Salmonella enterica serovar Heidelberg ( Salmonella heidelberg ): Implications for organism tracing
Author(s) -
Harvey Deborah,
Harrington Carolyn,
Heuzenroeder Michael W.,
Murray Christopher
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06118.x
Subject(s) - lysogenic cycle , cosmid , phage typing , biology , bacteriophage , lysogen , phagemid , salmonella , restriction fragment length polymorphism , microbiology and biotechnology , virology , genetics , serotype , dna , gene , bacteria , escherichia coli , polymerase chain reaction
A phage typing system using a group of 11 closely related phage (as judged by Southern analysis and restriction fragment length polymorphism analysis) was able to distinguish at least six phage types in Salmonella heidelberg of human and animal origin. Restriction fragment length polymorphism analysis using cosmid probes from S. heidelberg confirmed that most S. heidelberg isolates belong to a single ‘clonal’ group. Southern analysis using DNA isolated from each of the testing phage group showed that phage types 4, 5 and 6 carry closely related endogenous or lysogenic phage. Induction of a lysogenic phage Hlp‐4 (Heidelberg lysogenic phage) from type 4 could become lysogenic and convert phage types 1 and 3 to phage type 4 and phage type 5 to a non‐typable phenotype.