Open AccessRapid, extensive and reversible vacuolation of Schizosaccharomyces pombe induced by amphotericin B
Author(s) -
Takeo Kanji,
Yarita Kyoko,
Yoshida Soichi,
Guan Hongquan,
Nishimura Kazuko,
Miyaji Makoto
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06113.x
Subject(s) - schizosaccharomyces pombe , amphotericin b , microgram , vacuole , yeast , chemistry , mycelium , microbiology and biotechnology , budding , biology , biochemistry , saccharomyces cerevisiae , botany , in vitro , antifungal , cytoplasm
The fission yeast Schizosaccharomyces pombe has no large vacuoles under normal growth conditions, although budding yeasts usually have large central vacuoles. The minimum inhibitory concentration of amphotericin B to S. pombe was 0.5 μg ml −1 ; treatment with 0.2 μg ml −1 for 20 min induced rapid and extensive vacuolation in S. pombe exponential phase cells. Growth rate of the cells with 0.2 μg ml −1 amphotericin B was much reduced for 6 h, showing extensive vacuolation. Vacuolation in itself was not fatal: on removal of the drug, most cells recovered gradually and eventually multiplied.