Open AccessThermoalkalophilic lipase‐producing Bacillus selected by continuous cultivation
Author(s) -
Emanuilova E.,
Kambourova M.,
Dekovska M.,
Manolov R.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06107.x
Subject(s) - lipase , laboratory flask , thermophile , strain (injury) , bacillus (shape) , food science , bacteria , chemistry , enzyme , microbiology and biotechnology , enzyme assay , biology , biochemistry , genetics , anatomy
Several lipase‐producing thermophilic bacteria were isolated by continuous culture from samples collected near Bulgarian hot springs. Most of them had lipase activity of about 0.5 U ml −1 when activated in shaken flasks. Three strains, Gram‐positive sporeforming rods, possess higher enzyme activity in a Tween‐80 containing medium. The highest lipase activity (2.0–3.0 U ml −1 ) was observed in the newly‐isolated thermoalkalophilic Bacillus sp. strain MC7.