Open AccessTransformation of Gram‐positive microorganisms with the Gram‐negative broad‐host‐range cosmid vector pJRD215
Author(s) -
Hermans Jo,
Suy Ignace M.L.,
Bont Jan A.M.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06099.x
Subject(s) - biology , microbiology and biotechnology , mycobacterium smegmatis , transformation (genetics) , cosmid , kanamycin , bacillus subtilis , mycobacterium , lactococcus lactis , corynebacterium , brevibacterium , bacteria , mycobacterium tuberculosis , microorganism , genetics , gene , antibiotics , tuberculosis , medicine , lactic acid , pathology
A number of Gram‐positive bacterial species of biotechnological importance were screened for the expression of the Gram‐negative broad‐host‐range cosmid pJRD215, previously shown to be expressed in Mycobacterium aurum and Mycobacterium smegmatis . As judged from acquired kanamycin or streptomycin resistance, pJRD215 was expressed in five out of ten species tested: Bacillus subtilis, Brevibacterium sp., Corynebacterium glutamicum, Lactococcus lactis subsp. lactis and Mycobacterium parafortuitum .