Open AccessAmino acid sequence of heat‐labile enterotoxin from chicken enterotoxigenic Escherichia coli is identical to that of human strain H 10407
Author(s) -
Inoue Takashi,
Tsuji Takao,
Koto Michio,
Imamura Seiji,
Miyama Akio
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06092.x
Subject(s) - enterotoxin , heat stable enterotoxin , plasmid , strain (injury) , gene , heat labile enterotoxin , microbiology and biotechnology , escherichia coli , dna , biology , chemistry , biochemistry , anatomy
The DNA sequence of heat‐labile enterotoxin from the chicken enterotoxigenic Escherichia coli 21d strain was determined by direct dideoxy sequencing of polymerase chain reaction (PCR)‐amplified DNA and was compared with those of heat‐labile enterotoxins from porcine and human enterotoxigenic E. coli strains EWD 299 and H 10407. The structural genes of the A and B subunits of chicken heat‐labile enterotoxin were identical to those of human heat‐labile enterotoxin from the human H 10407 strain. Moreover, 67 base pairs of the upstream and 60 base pairs of the downstream region of the chicken heat‐labile enterotoxin gene were also identical to those of the human heat‐labile enterotoxin from strain H 10407. However, the patterns of plasmids from the 21d and H 10407 strains were different. The 21d strain had no band corresponding to the 42‐MDa plasmid of the H10407 strain encoding the heat‐labile enterotoxin gene but it had a smaller plasmid. These data suggest that although the DNA sequence of chicken heat‐labile enterotoxin is identical to that of human heat‐labile enterotoxin, the plasmid encoding the chicken heat‐labile enterotoxin gene in the chicken might be different from that encoding the human heat‐labile enterotoxin gene in the H10407 strain.