Open AccessAndrogen binding proteins in Cochliobolus lunatus
Author(s) -
KastelicSuhadolc Tatjana,
Lenasi Helena
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06084.x
Subject(s) - androstenedione , androgen , androgen binding protein , chemistry , dihydrotestosterone , cytosol , binding protein , estrone , testosterone (patch) , medicine , endocrinology , binding site , steroid , biochemistry , biology , enzyme , hormone , gene
Testosterone and 4‐androstene‐3,17‐dione (androstenedione) binding proteins were found in the cytosol of Cochliobolus lunatus , a filamentous fungus. The maximal androgen binding occurrd by 120 min at 0°C. Androgen binding proteins were degraded by protease treatment and at high temperature. The mean K d determined for testosterone binding protein (TBP) by Scatchard analysis was 4.5 nM and the maximum capacity B max was 120 fmol/mg protein; for androstenedione binding protein (ABP) the K d was 8 nM and B max was 390 fmol/mg protein. Steroid binding specificity for TBP, in order of decreasing affinity, was the following: androstenedione, progesterone, dihydrotestosterone, 17β‐estradiol and estrone. The same order was found for ABP.