Open AccessGenetic interrelationships of saccharolytic Clostridium botulinum types B, E and F and related clostridia as revealed by small‐subunit rRNA gene sequences
Author(s) -
Hutson R.A.,
Thompson D.E.,
Collins M.D.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06081.x
Subject(s) - clostridia , clostridium botulinum , biology , clostridiaceae , 16s ribosomal rna , phylogenetic tree , microbiology and biotechnology , clostridiales , ribosomal rna , gene , sequence analysis , phylogenetics , genetics , bacteria , toxin
The phylogenetic interrelationships of saccharolytic C. botulinum types B, E and F together with eleven other saccharolytic clostridia were examined by 16S rRNA gene sequencing. Comparative analysis of the sequence data revealed that the saccharolytic C. botulinum types B, E and F were highly related and represents a single genetic group. Strains of C. barati and C. butyricum that produce botulinal neurotoxin revealed almost 100% 16S rRNA sequence identity with their respective non‐toxigenic counterparts and were phylogenetically distinct from saccharolytic C. botulinum (types B, E and F). Proteolytic C. botulinum type F was shown to be phylogenetically remote from the saccharolytic C. botulinum group. The implications of the sequence data for the taxonomy of the C. botulinum complex are discussed.