Open AccessChromatographic determination of optical configuration of 3‐hydroxy fatty acids composing microbial surfactants
Author(s) -
Nakagawa Yoji,
Matsuyama Tohey
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06080.x
Subject(s) - serratia marcescens , chromatography , chemistry , glycolipid , hydrolysate , high performance liquid chromatography , fatty acid , organic chemistry , biochemistry , escherichia coli , hydrolysis , gene
The chromatographic determination of the optical configuration of 3‐hydroxy fatty acids of microbial surfactants was achieved in chiral high pressure liquid chromatography (HPLC) by injecting 3,5‐dinitroaniline‐derivatives of crude hydrolysates (less than 1 mg). Serrawettin W2, a surface‐active cyclodepsipeptide of Serratia marcescens , was shown to contain d ‐3‐hydroxydecanoic acid. Rubiwettin R1 and RG1, surface active glycolipid and linked fatty acids of Serratia rubidaea , were shown to contain d ‐3‐hydroxytetradecanoic acid and d ‐3‐hydroxydecanoic acid. The new method does not require purified sample or authentic optical isomers, and could be useful in the structural analysis of microbial lipids.