Open AccessA method for the isolation of RNA from Streptococcus salivarius and its application to the transcriptional analysis of the gtfJK locus
Author(s) -
Simpson Christine L.,
Giffard Philip M.,
Jacques Nicholas A.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06079.x
Subject(s) - streptococcus salivarius , biology , gene , terminator (solar) , glucosyltransferase , rna , transcription (linguistics) , rnase p , genetics , microbiology and biotechnology , streptococcus , bacteria , ionosphere , linguistics , philosophy , physics , astronomy
The glucosyltransferases from oral streptococci cleave sucrose and polymerize the glucose moieties. In Streptococcus salivarius ATCC 25975, two glucosyltransferase‐encoding genes, gtfJ and gtfK , are closely linked and transcribed in the same direction. A procedure for the isolation of intact RNA from this organism was devised. The procedure incorporated a high‐temperature mutanolysin treatment and selective precipitation by LiCl. The RNA was subject to Northern hybridization and RNase protection assays and it was concluded that the two genes are transcribed separately. A potential factor‐independent transcription terminator was located in the intergenic region.