Open AccessChemical structure of the 2‐keto‐3‐deoxyoctonate region of lipopolysaccharide isolated from Porphyromonas (Bacteroides) gingivalis
Author(s) -
Kumada Hidefumi,
Kondo Seiichi,
Umemoto Toshio,
Hisatsune Kazuhito
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06076.x
Subject(s) - porphyromonas gingivalis , lipopolysaccharide , chemistry , derivatization , dephosphorylation , polysaccharide , bacteroides , bacteroidaceae , hydrolysis , biochemistry , microbiology and biotechnology , stereochemistry , mass spectrometry , bacteria , chromatography , phosphorylation , biology , phosphatase , genetics , endocrinology
Structural analysis of the 2‐keto‐3‐deoxyoctonate region of lipopolysaccharide (LPS) isolated from Porphyromonas (Bacteroides) gingivalis was carried out. The substitution of the polysaccharide portion on the KDO was determined by gas chromatography/mass spectrometry of the product obtained by sequential derivatization of the LPS, including dephosphorylation, permethylation, carboxyl reduction, partial hydrolysis, carbonyl reduction, complete hydrolysis and O ‐acetylation. It was revealed that the KDO carries the polysaccharide on its position C5 and is phosphorylated on either position C7 or C8, although its exact position is not determined. The structure of the KDO region of P. gingivalis LPS in Gram‐negative bacterial LPS had not hitherto been elucidated.