Open AccessArginine utilization by Mycoplasma fermentans is not regulated by glucose metabolism: A 13 C‐NMR study
Author(s) -
Olson Lyn D.,
Renshaw Carolyn A.,
Rottem Shlomo,
Boal Jila H.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06072.x
Subject(s) - arginine , citrulline , biochemistry , metabolism , lactic acid , chemistry , hydrolysis , amino acid , biology , bacteria , genetics
13 C‐NMR studies on the effect of glucose metabolism on arginine hydrolysis in Mycoplasma fermentans cells have been performed using a continuous perfusion technique. With this procedure we were able to show, in the presence of glucose, the rapid accumulation of lactic acid and, in the presence of arginine, the formation of citrulline that is apparently further metabolized. As the accumulation of lactate and the breakdown of arginine were observed in the simultaneous presence of both substrates, it is suggested that the glucose utilization has little or no effect on the deimination of arginine to citrulline.