Open AccessUsing fusions with luxAB from Vibrio harveyi MAV to quantify induction and catabolite repression of the xyl operon in Staphylococcus carnosus TM300
Author(s) -
Sizemore Christine,
Geiβdörfer Walter,
Hillen Wolfgang
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06047.x
Subject(s) - vibrio harveyi , operon , bioluminescence , luciferase , catabolite repression , microbiology and biotechnology , biology , quorum sensing , staphylococcus xylosus , vibrio , vibrionaceae , bacteria , xylose , gene , biochemistry , staphylococcus aureus , staphylococcus , escherichia coli , virulence , fermentation , genetics , transfection , mutant
The luxA,B genes from the Gram‐negative marine bacterium Vibrio harveyi MAV were used in Staphylococcus carnosus TM300 as a reporter system for regulated expression of xylose utilization. The luciferase genes were fused to the xyl operon from Staphylococcus xylosus C2a. Expression of bioluminescence was induced through addition of xylose and repressed in the presence of glucose. A method to quantitate bioluminescence directly from the culture is described.