Open AccessFoot‐and‐mouth disease virus proteinase 3C inhibits translation in recombinant Escherichia coli
Author(s) -
Marquardt Otfried
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06043.x
Subject(s) - foot and mouth disease virus , escherichia coli , translation (biology) , ribosome , biology , gene expression , recombinant dna , microbiology and biotechnology , protein biosynthesis , gene , messenger rna , transcription (linguistics) , virus , virology , biochemistry , rna , linguistics , philosophy
Escherichia coli cultures do not survive the expression of recombinant foot‐and‐mouth disease virus proteinase 3C. This effect is ascribed to degradation of bacterial protein(s), as concluded from the observation of gradual cessation of gene expression upon induction of 3C expression. Most likely, translation inhibition is the cause of bacterial death, as (i) cell‐free translation of the 3C gene was restored by additional bacterial ribosomes, (ii) ribosomes from proteinase 3C‐producing cells differed from normal ones by a reduced content of protein S18, and (iii) transcription was not inhibited.