Open AccessOptimization of liquid culture medium for commercial production of Colletotrichum truncatum *
Author(s) -
Silman Robert W.,
Nelsen Terry C.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06042.x
Subject(s) - production (economics) , liquid culture , colletotrichum , liquid medium , microbiology and biotechnology , chemistry , biology , horticulture , botany , chromatography , economics , macroeconomics
A commercial type liquid culture medium for the production of Colletotrichum truncatum NRRL 13737, a pathogen of the weed hemp sesbania, was developed. The concentrations of culture ingredients: 20 g glucose 1 −1 , 2.5 g Tastone yeast extract 1 −1 , 7.5 g Pharmamedia 1 −1 are optimum for batch cultures. Initial glucose concentration determined total dry weight which was largely composed of mycelia: Pharmamedia concentration influenced the yield and rate of spore production; Tastone 154 yeast extract apparently supplied a nutritional factor which also affected the yield and rate of spore production but to a lesser extent. Batch culture with the described media yielded 6.2×10 7 spores per ml in three days. Studies of spore recovery, drying and storage can now be conducted with a commercial type culture medium.