
High larvicidal activity of intact recombinant cyanobacterium Anabaena sp. PCC 7120 expressing Gene 51 and Gene 42 of Bacillus sphaericus sp. 2297
Author(s) -
Xudong Xu,
Renqiu Kong,
Yuxiang Hu
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06037.x
Subject(s) - bacillus sphaericus , anabaena , biology , microbiology and biotechnology , bacillus thuringiensis , shuttle vector , cyanobacteria , recombinant dna , gene , bacillales , vector (molecular biology) , bacteria , genetics , bacillus subtilis
Gene 51 and Gene 42 of Bacillus sphaericus 2297 were expressed in the nitrogen‐fixing filamentous cyanobacterium Anabaena sp. 7120 on a shuttle vector. Intact recombinant cells showed high toxicity against larvae of Culex pipiens in laboratory and field tests, and lower toxicity against larvae of Anopheles sinensis . Although unselective culture could decrease the mosquitocidal activity, the genetically engineered Anabaena open up new possibilities for the use of cyanobacteria for mosquito control.