Open AccessThe cloning and nucleotide sequence of a Corynebacterium glutamicum 3‐deoxy‐ d ‐ arabinoheptulosonate‐7‐phosphate synthase gene
Author(s) -
Chen ChianChi,
Liao ChiiCherng,
Hsu WenHwei
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06034.x
Subject(s) - corynebacterium glutamicum , cloning (programming) , atp synthase , nucleic acid sequence , gene , corynebacterium , biology , sequence (biology) , nucleotide , carbamoyl phosphate synthetase , molecular cloning , genetics , biochemistry , microbiology and biotechnology , chemistry , bacteria , peptide sequence , computer science , programming language
The aro gene of Corynebacterium glutamucum CCRC 18310 encoding 3‐deoxy‐ d ‐arabinoheptulosonate‐7‐phosphate (DAHP) synthase was isolated by complementation of a DAHP synthase‐deficient mutant of Escherichia coli AB3257. The specific activity of DAHP synthase was increased four‐fold in a C. glutamicum strain harboring the cloned aro gene. The complete nucleotide sequence of the aro gene and 5′ and 3′ flanking regions has been determined. The sequence contained an open reading frame of 368 codons, from which a protein with a molecular mass of 39 340 Da could be predicted. The deduced amino acid sequence shows high identity with the aro gene products of E. coli and Salmonella typhimurium .