Open AccessRestriction fragment length polymorphism analysis of Fusobacterium necrophorum using a novel repeat DNA sequence and a 16S rRNA gene probe
Author(s) -
Hodgson A.L.M.,
Nicholson L.A.,
Doran T.J.,
Corner L.A.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06031.x
Subject(s) - biology , fusobacterium necrophorum , restriction fragment length polymorphism , genetics , sequence analysis , microbiology and biotechnology , ribosomal rna , ribosomal dna , gene , terminal restriction fragment length polymorphism , repeated sequence , restriction fragment , homology (biology) , polymerase chain reaction , phylogenetics , genome
A repeated DNA sequence was isolated from Fusobacterium necrophorum biotype AB, strain FnS1. The repeated sequence shared considerable homology with the transposase gene from the Pseudomonas syringiae insertion sequence IS801. The repeat sequence was used together with a 16S ribosomal RNA gene probe to type F. necrophorum isolates using restriction fragment length polymorphisms. The probes revealed differences between several clinical isolates and will be useful tools to study the epidemology of ovine foot abscess and other diseases caused by F. necrophorum .