Open AccessProduction of γ‐linolenic acid from the marine green alga Chlorella sp. NKG 042401
Author(s) -
Miura Yuki,
Sode Koji,
Nakamura Noriyuki,
Matsunaga Naoki,
Matsunaga Tadashi
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06024.x
Subject(s) - gamma linolenic acid , salinity , fatty acid , linolenic acid , chlorella , food science , urea , chemistry , algae , botany , chlorophyta , biochemistry , biology , polyunsaturated fatty acid , linoleic acid , ecology
Abstract γ‐Linolenic acid (GLA) production using a high GLA producing marine green alga, Chlorella sp. NKG 042401, was studied. GLA was presented in the galactolipid fraction (37.9%/total fatty acids). The effects of growth conditions on GLA production were studied. Optimum salinity for GLA production was 5 g 1 −1 , at which salinity the highest cell concentration was achieved, resulting in a 1.6‐fold increase in GLA productivity. Total fatty acid, however, was not drastically affected by change of salinity. Nitrogen starvation decreased the ratio of unsaturated fatty acids, and consequently GLA ratio in total fatty acid decreased. The urea adduct method was used to concentrate GLA from crude extract. As a result, after 5 sequential concentration procedures, GLA was concentrated 5‐fold with a yield of 49%.