Open AccessEnergy conservation by pyrroloquinoline quinol‐linked xylose oxidation in Pseudomonas putida NCTC 10936 during carbon‐limited growth in chemostat culture
Author(s) -
Hardy Guy P.M.A.,
Joost Teixeira de Mattos M.,
Neijssel Oense M.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06012.x
Subject(s) - xylose , chemostat , pseudomonas putida , pentose , biochemistry , energy source , chemistry , periplasmic space , biology , bacteria , organic chemistry , enzyme , fermentation , coal , escherichia coli , gene , genetics
When grown in carbon source‐limited chemostat cultures with lactate or glucose as the carbon and energy source and xylose as an additional source of reducing equivalents, Pseudomonas putida NCTC 10936 oxidized xylose to xylonolactone and xylonate. No other products were formed from this pentose, nor was it incorporated into biomass. The presence of xylose in these cultures resulted in higher Y glucose and Y lactate values as compared to cultures without xylose indicating that biologically useful energy was conserved during the periplasmic oxidation of xylose. As the Y 0 values for growth on glucose or on lactate alone were equal to the Y 0 values with xylose as co‐substrate, it is concluded that for flucose‐ or lactate‐limited growth energy conservation by PQQH 2 oxidation is as efficient as by NADH 2 oxidation.