Open AccessIn vitro translation of archaeal natural mRNAs at high temperature
Author(s) -
Ruggero Davide,
Creti Roberta,
Londei Paola
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06009.x
Subject(s) - sulfolobus solfataricus , thermophile , ribosome , archaea , in vitro , sulfolobus , translation (biology) , biology , biochemistry , protein biosynthesis , chemistry , rna , messenger rna , enzyme , gene
Efficient in vitro translation of archaeal natural mRNAs at high (75°C) temperature has been achieved by employing either crude cell lysates or purified ribosomes and soluble proteins of the extreme thermophile Sulfolobus solfataricus . The features of the system are described.