Open AccessIdentification of Streptococcus mutans antigen D as the HPr component of the sugar‐phosphotransferase transport system
Author(s) -
Sutcliffe Iain C.,
Hogg Stephen D.,
Russell Roy R.B.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06005.x
Subject(s) - streptococcus mutans , antiserum , pep group translocation , antigen , phosphotransferase , chemistry , biochemistry , polyacrylamide gel electrophoresis , microbiology and biotechnology , blot , biology , bacteria , enzyme , phosphoenolpyruvate carboxykinase , gene , genetics
Extraction of Streptococcus mutans with the detergents HECAMEG and lauroyl sarcosinate preferentially extracted antigen D, previously identified as a low molecular mass wall‐associated protein. Western blotting with specific antisera was used to demonstrate that this antigen is the HPr component of the sugar‐phosphotransferase transport system. Non‐denaturing electrophoresis indicated that HECAMEG selectively extracted only one of the two forms of HPr. It is suggested that this form of HPr may have a specific cell surface location.