Open AccessScreening of thermotolerant yeasts as producers of superoxide dismutase
Author(s) -
Nedeva T.S.,
Savov V.A.,
KujumdzievaSavova A.V.,
Davidov E.R.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb06002.x
Subject(s) - superoxide dismutase , kluyveromyces lactis , thermostability , yeast , enzyme , mesophile , biochemistry , pichia , dismutase , food science , biology , kluyveromyces , chemistry , saccharomyces cerevisiae , bacteria , pichia pastoris , recombinant dna , genetics , gene
A screening procedure for highly thermostable yeast superoxide dismutase was developed. Growth yields at various temperatures were estimated for ten mesophilic and thermotolerant strains, belonging to the genera Saccharomyces, Kluyveromyces and Pichia . Higher yields at 45°C were obtained for K. lactis 90‐3 and 90‐4. A correlation between the ability to grow at higher temperature and the thermostability of the superoxide dismutase enzyme synthesized was observed. A comparison of the operational stability of the superoxide dismutase of all tested strains suggests that the enzyme of K. lactis strains was more thermostable than that of the other tested microorganisms.