Open AccessA yeast gene for trehalose‐6‐phosphate synthase and its complementation of an Escherichia coli otsA mutant
Author(s) -
McDougall John,
Kaasen Inga,
Str?m Arne R.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb05998.x
Subject(s) - complementation , trehalose , escherichia coli , atp synthase , mutant , biochemistry , biology , sugar phosphates , gene , saccharomyces cerevisiae , yeast , protein fragment complementation assay , microbiology and biotechnology , phosphate
A Saccharomyces cerevisiae gene for trehalose‐6‐phosphate synthase (TPS1) was sequenced. The gene appeared to code for a protein of 495 amino acid residues, giving the protein a molecular mass of 56 kDa. The TPS1 gene was able to restore both osmotolerance and trehalose accumulation during salt stress in an Escherichia coli strain mutated in the otsA gene encoding trehalose‐6‐phosphate synthase. Complementation studies with E. coli galU mutants showed that the TPS1‐encoded trehalose‐6‐phosphate synthase is UDP‐glucose‐dependent. Sequence analysis and data base searches showed that TPS1 is allelic to GGS1, byp1, cif1 and fdp1 . A possible gene for trehalose‐6‐phosphate synthase in Methanobacterium thermoautotrophicum was identified.