Open AccessEvolution of tubulin isoforms during the sporophytic development of Allomyces arbuscula
Author(s) -
Schönenberger Isabelle,
Turian Gilbert
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb05996.x
Subject(s) - gene isoform , zoospore , biology , mycelium , acetylation , oomycete , tubulin , microbiology and biotechnology , phycomycetes , monoclonal antibody , isozyme , microtubule , hypha , botany , biochemistry , antibody , enzyme , gene , genetics , immunology , spore , inoculation
Tubulins extracted from the sporophytic developmental stages of Allomyces arbuscula have been characterised by one‐ and two‐dimensional SDS‐PAGE gels immunoblotted with monoclonal antibodies as α‐, acetylated α‐ ( M r 57 kDa both) and β‐ ( M r 55 kDa) isoforms. The zoosporangial isoforms could be characterised only when precautions were taken to inhibit the strong tubulin proteolytic activity at this stage. The zoospores and zoosporangia contained greater amounts of the acetylated α‐ and β‐isoforms than the mycelium, while the non acetylated α‐isoform was present in greater quantity in the mycelium than in the zoospores or zoosporangia.