Open AccessProduction and characterisation of a Legionella pneumophila specific monoclonal antibody
Author(s) -
Lever M. Stephen
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb05995.x
Subject(s) - legionella pneumophila , legionella , monoclonal antibody , epitope , microbiology and biotechnology , bacterial outer membrane , peptidoglycan , biology , antigen , antibody , bacteria , virology , escherichia coli , biochemistry , immunology , gene , genetics
To better define the antigenic structure of the outer cell membranes of Legionellae, a panel of 6 monoclonal antibodies was raised against partially purified outer membranes of Legionella pneumophila serogroup 1, Corby strain. This study describes the purification and characterization of one of these monoclonal antibodies reacting with a 135‐kDa protein, which was shown to be common to all 14 serogroups of Legionella pneumophila . It shows no cross‐reactivity with 20 other Legionella species, or 9 other Gram‐negative species tested by SDS‐PAGE and Western blotting procedures. The epitope would appear to be predominantly surface exposed and, from preliminary detergent extraction studies, not peptidoglycan‐associated.