Open AccessDevelopment of a selective and sensitive polymerase chain reaction assay for the detection of Mycoplasma pirum
Author(s) -
Grau Odile,
Kovacic Rémi,
Griffais Rémy,
Montagnier Luc
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb05984.x
Subject(s) - mollicutes , polymerase chain reaction , biology , microbiology and biotechnology , dna , mycoplasma , dna polymerase i , escherichia coli , bacteria , mycoplasmataceae , bacillus subtilis , mycoplasma pneumoniae , ribosomal rna , polymerase , gene , biochemistry , genetics , reverse transcriptase , pneumonia , history , archaeology
A new assay using the polymerase chain reaction to amplify a 173‐nucleotide DNA fragment within the 16S ribosomal RNA gene of Mycoplasma pirum has been developed. The assay selectively amplified DNA from all strains of M. pirum tested with a high level of sensitivity, even in a context of human DNA. DNA from other mollicute species, including those closely related to M. pirum , from bacteria phylogenetically close to mollicutes ( Clostridium innocuum, C. ramosum and Bacillus subtilis ), from Escherichia coli and from human peripheral blood mononuclear cells, did not produce the amplified DNA product specific for M. pirum .