Open AccessPhysical analysis of in vivo pCI301 fusion plasmids in Lactococcus lactis subsp. lactis
Author(s) -
Hayes Finbarr,
Daly Charles,
Fitzgerald Gerald F.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb05979.x
Subject(s) - lactococcus lactis , plasmid , biology , microbiology and biotechnology , recombinant dna , genetics , bacteria , gene , lactic acid
In vivo fusion plasmids identified following conjugative mobilization of pCI301, the 75‐kilobase (kb) lactose‐proteinase plasmid of Lactococcus lactis subsp. lactis UC317, were characterized. These plasmids (95 kb) were generated from fusion‐deletion events involving pCI301 and the 38‐kb UC317‐derived cryptic plasmid, pCI303. Recombinant plasmids were separable into distinct classes based on their associated phenotypes and restriction maps. The formation of pCI301: : pCI303 composite plasmids within strain UC317 was also demonstrated.