Open AccessCloning and characterization of heat‐inducible promoters of Bacillus subtilis
Author(s) -
Völker Uwe,
Riethdorf Sabine,
Winkler Anett,
Weigend Bettina,
Fortnagel Peter,
Hecker Michael
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb05978.x
Subject(s) - bacillus subtilis , promoter , sigma factor , rna polymerase , transcription (linguistics) , gene , biology , escherichia coli , microbiology and biotechnology , dna , heat shock protein , genetics , gene expression , bacteria , linguistics , philosophy
Heat‐inducible DNA fragments of Bacillus subtilis were cloned with two different promoter probe vectors. The increased synthesis of the reporter enzymes seemed to be due to a transient increase in the transcription of the encoding genes. The structures of the heat‐sensitive promoters resembles the consensus sequence of promoters recognized by the vegetative form of RNA polymerase of B. subtilis . Our results support data in literature that the heat shock response of B. subtilis is regulated by a different mechanism than in Escherichia coli , where alternative sigma factors direct the transcription of heat shock genes.