Open AccessAnalysis of the Spirochaeta aurantia flaA gene and transcript
Author(s) -
Parales Juan,
Greenberg E.P.
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb05971.x
Subject(s) - open reading frame , gene , biology , terminator (solar) , genetics , peptide sequence , nucleic acid sequence , base pair , start codon , stop codon , microbiology and biotechnology , messenger rna , ionosphere , physics , astronomy
The flaA gene, which codes for the Spirochaeta aurantia flagellar filament outer layer polypeptide, FlaA, was cloned, sequenced and analysed. The gene appears to be transcribed into a monocistronic mRNA from a σ 70 ‐like promoter. The translational start is 31 base pairs after the start of the transcript, the open reading frame is 1011 base pairs, and a rho ‐independent‐like transcription terminator sequence begins about 19 base pairs after the translational stop codon. The deduced amino acid sequence of the S. aurantia FlaA showed 40% identity with the Treponema pallidum FlaA, but these polypeptides did not show a significant similarity to other polypeptides for which sequence information was available.