Open AccessCovalently immobilized DNA plate for luminometric DNA‐DNA hybridization to identify viridans streptococci in under 2 hours
Author(s) -
Adnan Shatha,
Li Na,
Miura Hiroaki,
Hashimoto Yasuhiro,
Yamamoto Hiroyuki,
Ezaki Takayuki
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb05949.x
Subject(s) - dna , viridans streptococci , dna–dna hybridization , microbiology and biotechnology , chemiluminescence , hybridization probe , covalent bond , nucleic acid thermodynamics , biology , streptococcus , chemistry , bacteria , biochemistry , genetics , chromatography , base sequence , organic chemistry
Single‐stranded chromosomal DNA was covalently bound to a microdilution plate and used for quantitative DNA‐DNA hybridization. After 30 min, hybridized DNA was quantitatively detected by alkaline phosphatase and a chemiluminescent substrate. This method was successfully used for the rapid identification of viridans streptococci.