Open AccessCloning of the replication region on the bacteriocinogenic plasmid pRJ9 of Staphylococcus aureus
Author(s) -
Oliveira Selma Soares,
Araújo Sandra Amaral,
Freire Bastos Maria do Carmo
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb05946.x
Subject(s) - replicon , plasmid , bacteriocin , biology , staphylococcus aureus , cloning (programming) , microbiology and biotechnology , molecular cloning , recombinant dna , clone (java method) , cloning vector , genetics , vector (molecular biology) , gene , bacteria , peptide sequence , computer science , programming language
A 5.8‐kb Cla I fragment of pRJ9, a bacteriocinogenic plasmid of Sphylococcus aureus , was cloned in the unique Cla I site of pRJ5. The recombinant plasmid obtained, pRJ23, failed to confer bacteriocin production and immunity to bacteriocin on host cells. The cloned fragment was shown to contain the complete replicon of pRJ9. Attempts to clone the 4.4‐kb Cla I fragment of pRJ9 were unsuccessful, apparently due to the inactivation of the basic replicon of the cloning vector. Therefore, plasmid pRJ5 cut at its Cla I site appears to be a suitable vector for cloning replication regions of plasmids that cab replicate in S. aureus .