Open AccessLocation of fructose in lipopolysaccharide isolated from 01 Vibrio cholerae NIH 41R
Author(s) -
Kondo Seiichi,
Haishima Yuji,
Hisatsune Kazuhito
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb05940.x
Subject(s) - lipopolysaccharide , vibrio cholerae , fructose , residue (chemistry) , chemistry , mutant , microbiology and biotechnology , biochemistry , strain (injury) , monosaccharide , sugar , derivative (finance) , bacteria , stereochemistry , biology , anatomy , genetics , gene , financial economics , economics , endocrinology
Fructose, a rarely occurring sugar constituent of Gram‐negative bacterial lipopolysaccharides (LPS), is distributed ubiquitously in LPS of 01 Vibrio cholerae so far examined, but its location in LPS has not hitherto been elucidated. It was found that hydrazinolysis of LPS successfully affords a derivative retaining virtually all the fructose of intact LPS, but no ester‐bound phosphate. Structural analysis carried out on the LPS derivative prepared by the hydrazinolysis of R‐type LPS isolated from a rough mutant strain (NIH 41R) of 01 V. cholerae NIH 41 (Ogawa) revealed that the fructose is present as a non‐reducing terminal residue bound to position C‐6 of a glucose residue in the core region. This finding is considered to exclude the possibility that, in the LPS of 01 V. cholerae , the fructose is present in the region of the inner core in place of 2‐keto‐3‐deoxyoctonate.