Open AccessBiophysical characterization of Vitrio El Tor typing phage e5
Author(s) -
Basu Rina,
Ghosh A.N.,
Dasgupta S.,
Ghosh Amit
Publication year - 1993
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1993.tb05928.x
Subject(s) - vibrio cholerae , el tor , hpaii , restriction enzyme , biology , bacteriophage , microbiology and biotechnology , phage typing , dna , typing , lysis , molecular mass , chemistry , genetics , bacteria , escherichia coli , enzyme , biochemistry , gene , gene expression , dna methylation
Vibrio cholerae typing phage e5, which can lyse only the El Tor strains of V. cholerae , was characterized. The phage had a polyhedral head 51 nm in diameter and a short tail 13 nm in length. It contained 13 structural polypeptides, with the molecular mass of the major component being 50 kDa. Phage chromosome comprised a 38.5‐kb linear double‐stranded DNA molecule with unique termini, as determined by restriction fragment analysis and electron microscopy, and had a G+C content of 35.5%. A physical map was constructed with the restriction endonucleases Hae II and Hpa II. Adsorption of the phage to its host followed a biphasic kinetics and its intracellular growth was characterized by a latent period of 15 min and a burst size of 100 particles per infected cell. The phage was found to be moderately thermotolerant.