Open AccessGrowth temperature‐dependent activity of glycerol dehydratase in Escherichia coli expressing the Citrobacter freundii dha regulon
Author(s) -
Daniel Rolf,
Gottschalk Gerhard
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb14053.x
Subject(s) - citrobacter freundii , regulon , cosmid , escherichia coli , glycerol , recombinant dna , biology , dehydratase , biochemistry , 1,3 propanediol , enterobacteriaceae , citrobacter , clone (java method) , chemistry , enzyme , dna , gene
Using the cosmid pWE15, a genomic library of Citrobacter freundii DNA in Escherichia coli ECL707 was prepared and screened for glycerol utilization. Six out of approximately 3000 clones were positive. One clone, harboring the recombinant cosmid pRD1, expressed glycerol dehydratase in high activity when grown at 28°C but not at 37°C. The growth temperature had little effect on the activity of the other enzymes encoded by the dha regulon. When the glycerol‐containing medium was supplemented with corrinoids, the recombinant E. coli strain produced 1,3‐propanediol in high amounts at 28°C.