Open AccessSpecific removal of chlorine from the ortho ‐position of halogenated benzoic acids by reductive dechlorination in anaerobic enrichment cultures
Author(s) -
Gerritse Jan,
Woude Bernard J.,
Gottschal Jan C.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb14052.x
Subject(s) - reductive dechlorination , chlorine , halogenation , chemistry , benzoic acid , chloride , enrichment culture , organic chemistry , reductive elimination , environmental chemistry , medicinal chemistry , biodegradation , catalysis , bacteria , biology , genetics
Anaerobic enrichment cultures catalysing the reductive dechlorination of chlorinated benzoic acids were obtained from three fresh‐water sediments collected from seven different locations. Sub‐cultures from these enrichments specifically removed ortho ‐substituted chlorine from 2,3,6‐, 2,3,5‐ and 2,4,6‐trichlorobenzoic acid, yielding chloride and 2,5‐, 3,5‐, and 2,4‐dichlorobenzoic acids, respectively. These reductive dehalogenations were stimulated by the addition of benzoate and/or volatile organic acids. In one of these enrichments dehalogenation of ortho ‐ and/or para ‐chlorine substituents was also observed from 2,3‐, 2,4‐, 2,5‐, and 3,4‐dichlorobenzoic acid, yielding 3‐ and 4‐chlorobenzoic acid, meta ‐chlorines was not observed in any of the enrichments.