Open AccessRegulation of nitrogen fixation in Azospirillum brasilense Sp7: Involvement of nifA, glnA and glnB gene products
Author(s) -
Liang Yao Yun,
Zamaroczy Miklos,
Arséne Florence,
Paquelin Annick,
Elmerich Claudine
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb14028.x
Subject(s) - azospirillum brasilense , mutant , nitrogen fixation , lac operon , glutamine synthetase , microbial inoculant , biology , gene , microbiology and biotechnology , bacteria , chemistry , escherichia coli , biochemistry , genetics , glutamine , amino acid
The expression of nifA‐, niH ‐ and nifB‐lacZ fusions was examined in different mutants of Azospirillum brasilense . Mutations in nifA, glnA and glnB severely impaired the expression of nifH ‐ and nifB‐lacZ fusions. By contrast, a nifA‐lacZ fusion was not affected in a nifA or a glnB background and was only partially impaired in glnA mutants. It is proposed that in A. brasilense , the P II protein and glutamine synthetase are involved in a post‐translational modification of NifA.