Open AccessUse of enzyme microassay to detect eosinophil potentiating activity of cytokines in sheep
Author(s) -
Stevenson Lesley M.,
Jones Douglas G.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05917.x
Subject(s) - eosinophil , eosinophil peroxidase , monoclonal antibody , granulocyte macrophage colony stimulating factor , eosinophil cationic protein , granulocyte , cytokine , macrophage , immunology , biology , granulocyte macrophage colony stimulating factor receptor , cell culture , colony stimulating factor , interleukin , microbiology and biotechnology , chemistry , antibody , biochemistry , macrophage colony stimulating factor , in vitro , haematopoiesis , genetics , stem cell , asthma
Microplate assays for eosinophil peroxidase (EPO) and arylsulphatase (EAS) have been established as indices of eosinophil survival/proliferation in sheep bone marrow cell (SBMC) cultures. Cell specificity was confirmed using density‐fractionated and differentially depleted SBMC populations. Several recombinant cytokines including interleukins 3 (IL‐3) and 5 (IL‐5), and granulocyte macrophage‐colony stimulating factor (GM‐CSF), but not macrophage‐CSF (M‐CSF), had demonstrable eosinophil‐potentiating activity on the basis of enhanced EPO and EAS activities in treated, compared with untreated, SBMC cultures. Effects of IL‐5 were abrogated in the presence of a specific monoclonal anti‐IL‐5 antibody. The results indicate that measurement of EPO and EAS in cultured SBMC offers a simple and effective method for detecting eosinophil potentiating activity in the ovine.