Open AccessSequential phospholipase activation in the stimulation of the neutrophil NADPH oxidase
Author(s) -
Watson Fiona,
Robinson John J.,
Edwards Steven W.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05908.x
Subject(s) - intracellular , nadph oxidase , phospholipase , biochemistry , stimulation , secretion , respiratory burst , phospholipase a2 , chemistry , protein kinase c , phospholipase a , biology , enzyme , endocrinology
Stimulation of human neutrophils with the chemotactic peptide fMet‐Leu‐Phe results in activation of a rapid, transient burst of oxidant secretion, which reaches a maximal rate by about 1 min after stimulation. This phase of oxidant secretion is then followed by intracellular oxidant production, which is detected by luminol chemiluminescence but not by assays such as cytochrome c reduction or scopoletin oxidation. The rapid phase of oxidant secretion requires increases in intracellular free Ca 2+ and phospholipase A 2 activity, but not the activities of phospholipase D or D or protein kinase C. In contrast, intracellular oxidant production requires the activities of phospholipase D and protein kinase C. A model is thus proposed suggesting the sequential activation of different phospholipases which activate oxidase molecules on the plasma membrane or else from the membranes of specific granules.