Open AccessThe HlyB/HlyD‐dependent secretion of toxins by Gran‐negative bacteria *
Author(s) -
Koronakis Vassilis,
Stanley Peter,
Koronakis Eva,
Hughes Colin
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05885.x
Subject(s) - periplasmic space , secretion , hemolysin , biology , escherichia coli , bacteria , context (archaeology) , cytoplasm , bacterial outer membrane , chromosomal translocation , gram negative bacteria , microbiology and biotechnology , biochemistry , genetics , virulence , gene , paleontology
Hemolysin (HlyA) and related toxins are secreted across both the cytoplasmic and outer membranes of Escherichia coli and other pathogenic Gram‐negative bacteria in a remarkable process which proceeds without a periplasmic intermediate. It is directed by an uncleaved C‐terminal targetting signal and the HlyD and HlyB translocator proteins, the latter of which are members of a transporter superfamily central to import and export of a wide range of substrates by prokaryotic and eukaryotic cells. Our mutational analyses of the HlyA targetting signal and definition for the first time of stages and intermediates in the HlyB/HlyD‐dependent translocation allow a discussion of the hemolysin export process in the wider context of protein translocation.