Open AccessSynthesis and degradation of polyhydroxyalkanoates in Alcaligenes eutrophus
Author(s) -
Doi Yoshiharu,
Kawaguchi Yasushi,
Koyama Naoyuki,
Nakamura Shigeo,
Hiramitsu Masaya,
Yoshida Yoshinori,
Kimura Hiroshi
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05827.x
Subject(s) - polyhydroxyalkanoates , cupriavidus necator , degradation (telecommunications) , polymer , biochemistry , chemistry , carbon fibers , enzyme , intracellular , alcaligenes , kinetics , polymerase , carbon source , stereochemistry , bacteria , organic chemistry , biology , pseudomonas , materials science , telecommunications , genetics , computer science , physics , quantum mechanics , composite number , composite material
The kinetics and mechanism of the synthesis and degradation of polyhydroxyalkanoates (PHA) in Alcaligenes eutrophus have been studied. PHA polymers were accumulated in the cells in nitrogen‐free mineral media containing various carbon substrates, and the accumulated PHA polymers were subsequently degraded after the carbon sources were exhausted. The number of PHA polymerase molecules per cell was determined to be 18,000. The kinetic data of poly(3‐hydroxybutyrate) (P(3HB)) synthesis indicated that about two molecules of d (−)‐3‐hydroxybutyryl‐CoA are added within 1 s into a propagating chain of P(3HB) on the active site of polymerase, and that the average lifetime of a propagating P(3HB) chain is about 1 h. The intracellular PHA depolymerase was suggested to be exo ‐type hydrolase. The pathway and regulation of PHA synthesis were studied using [5‐ 13 C]pentanoic acid as the sole carbon source.