Open AccessConstruction of a shuttle lacmZα‐based Escherichia coli‐actinomycetes vector containing the phage JHJ‐3 replicon
Author(s) -
Gaudreau Luc R.,
Gouill Christian Le,
Déry Claude V.
Publication year - 1992
Publication title -
fems microbiology letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.899
H-Index - 151
eISSN - 1574-6968
pISSN - 0378-1097
DOI - 10.1111/j.1574-6968.1992.tb05577.x
Subject(s) - shuttle vector , multiple cloning site , replicon , cloning vector , escherichia coli , plasmid , biology , cloning (programming) , streptomyces , vector (molecular biology) , molecular cloning , microbiology and biotechnology , genetics , gene , recombinant dna , bacteria , gene expression , computer science , programming language
Using the broad replicating range JHJ‐3 phage replicon, a shuttle vector for Escherichia coli and actinomycetes has been constructed. The vector, pOJ31, bears the lac Z α fragment allowing a blue/white gene cloning system. pOJ31 also contains a polylinker of 15 unique cloning sites and the phage T7 promoter. The vector has been used to stably express the mel gene from plasmid pIJ702 in Streptomyces lividans .